Hours, days, months? Any sort of reference on this would be great. Thank you.
The presence of oxygen and the lack of light induce catabolic breakdown and pheophytin is a significant label for it; test are avilable as in Porra et al. BBA bio 43036 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC158071/ Visual checks detect losses after a significant degradation has occurred. One may either use freezers or acetone fixation. The time course varies with vacuoles integrity in the range of days
Bekele - It is eastern white pine (Pinus strobus), so they are technically needle samples rather than true leaves.
Agree with Prof. Bongi. Checking Porra et al., 1989 leaf extract in acetone 80% can be measured for Chl content in 72 h.
The leaf samples can be stored atleast for a few days. At 4C the chlorophyllase will be less active. The chlorophyll can be extracted in either 80% acetone or DMF till atleast 4 days of storage.
Since leaves of Pine are needle shape and waxy there would be insignificant metabolic activity that could cause Chl degradation. In addition, pines synthesize chlorophyll in the dark (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC34732/). Hence, you can store your leaves for more days and extract the Chl. Look this helpful reference.
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