How long can I storage the collagen solutions?

More Kim Ui Hwan's questions See All

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Does soybean seed coat or cotyledon contain chlorophyl or flavonoids? What types? determined by paper chromatography? other methods (high school lab)?

Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.

25 July 2024 7,498 2 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

Can Document Analysis be used as the only method of research?

I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.

18 July 2024 4,218 0 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

In the case of Collagen Cell Carrier® membrane 34 mm for 6 well cell culture plate (Viscofan), can you suggest a paraffin embedding protocol?

I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.

05 June 2024 3,831 0 View

Why did my hydrogel suddenly become acidic without changing the conditions in which it was made?

I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...

29 May 2024 4,655 0 View

Can collagenase injection cause a reverse reaction leading to higher collagen deposition?

Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!

11 May 2024 2,931 0 View

How to understand tissue remodeling during engineered myocardial tissue culture?

I made a myocardial tissue using cardiomyocytes and type I collagen (3 mg/ml). During the culture process, the tissue remodeled and its width decreased. What factors influence the degree of...

10 May 2024 7,784 0 View

How to distinguish between new collagen fibers and old ones in an ECM gel using Masson's trichrome staining?

hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...

05 May 2024 2,297 0 View

How to extract collagen from bones specimen?

how to extract collagen from bones specimen?

27 April 2024 6,307 0 View

Protocol for Coating Plates with Collagen?

Does anyone have guidance on coating 96-well plates with collagen-I? Looking for a protocol. We are using Corning® Collagen I, Rat Tail, 100 mg.

22 April 2024 6,835 1 View

How can I measure fibrosis in a cardiac tissue?

Hi, I have used masson's trichrome staining and trying to analyze collagen staining.. How can we analyze it using Image J. Anyone have the macros available? Thanks

14 April 2024 5,607 2 View

Malcolm Nobre

Hello Kim Ui Hwan

If you are planning to store collagen solution for more than 2 weeks, I would suggest you make aliquots of collagen solution (of desired volume) for one time use and store at -20ºC. Storage of collagen solution at 4ºC is acceptable for a couple of days.

Best.

Kim Ui Hwan

Malcolm Nobre Thanks a lot. Your advice was very helpful.