I need to prepare glycerol stock for bacteria and the only available option is to keep at a -20 freezer.
I would also like to know this. I have to repair our -80 freezer, so it needs to be defrosted first. How long would bacterial glycerol stocks be stable at -20C?
dear all would you help me to find a scops journal in the field of chemical engineering with fees as I want to publish in a short time. thank you [email protected]
23 December 2020 6,883 1 View
What is your knowledge about the curriculum of excellence, and what are its features? Is it applied in your country? Your participation is great and appreciated
01 July 2020 2,103 16 View
What are the skills of the twenty-first century to be available to students at different levels of education? Your participation is of great importance to me
25 June 2020 705 29 View
Do you think that parental treatment during the ban period will affect an active role in the formation of the child's personality in psychological, social and personal terms?
16 May 2020 3,741 4 View
I have two-phase flow in the annular regime inside a pipe, laminar liquid and turbulent vapor in the core. Is it possible to assign two different models in ANSYS fluent for each phase (e.g....
24 March 2020 9,322 3 View
My master is a bout PV-connected to grid through VSC and i want to design an active power filter to cancel the injected harmonics from the nonlinear loads connected to the grid.
22 September 2019 1,682 3 View
Nickel, as a counter electrode, provides a constant baseline for liquid chomatography-amperometric detection while platinum fails to give a stable baseline, why? is that related to its atomic or...
02 April 2019 9,041 1 View
I am testing ISSR primers on plant DNA and the bands I get are very faint. I am adding 5 microliters to the wells from a 25 microliter PCR product, I have tried increasing the volume in the well...
09 February 2019 1,477 6 View
Hi, I was trying to assign the pressure and the mass flow rate at inlet for my case in Fluent, but after some research, I've found that u can't do that because it's mathematically over...
08 May 2018 4,658 1 View
Hi, I need to calculate the inertial and viscous resistance for porous material in Fluent. After checking the Fluent manual, I picked the Ergun equation. My question is how to find the values for...
06 May 2018 7,343 6 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.
21 February 2021 3,081 3 View
Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...
16 February 2021 3,348 1 View
11 February 2021 3,524 2 View
Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...
06 February 2021 2,300 3 View
Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...
06 February 2021 9,370 7 View
Hello everyone! I have one question. I have couple of solvents and all of them were pure when they were bought but after some months or years of storage after opening I am not 100% sure about...
03 February 2021 2,154 8 View
I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.
31 January 2021 2,311 3 View