I need to prepare glycerol stock for bacteria and the only available option is to keep at a -20 freezer.
I would also like to know this. I have to repair our -80 freezer, so it needs to be defrosted first. How long would bacterial glycerol stocks be stable at -20C?
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
In SPSS 25, I have a categorical variable which I would like to display in a frequency table. However, one of my categories was not selected by any of my respondents. I can generate a frequency...
31 May 2024 2,076 3 View
book or article
20 May 2024 2,488 3 View
most important insect parasites on the cotton mealybug?
19 January 2024 560 2 View
I am researching pronunciation and correct articulation among EFL students. I have a list of "problematic sounds" that students often struggle to pronounce, and I am trying to analyse those sounds...
24 December 2023 8,504 2 View
Why sodium hydroxide can cause increasing the fluorescence intensity for a fluorogenic reagent however acetone completely diminish fluorescence peak of the same reagent?
09 November 2023 3,715 1 View
What is the reason making the cyclic voltammograms looking so noisy like in the uploaded picture? is it a bad connection? something wrong with the instrument? or bad graphite source?
30 October 2023 162 3 View
Hello Scientific community, i'am seeking an oceanographer with expertise in satellite data analysis, specifically data from Sentinel-2A, to help me understand the reasons behind the excessive...
06 October 2023 8,909 0 View
What further capabilities and features can ROS offer? What is the scientific justification for using ROS rather than MATLAB?
03 October 2023 4,906 0 View
I have a mixture consisting of citric acid and calcium hydroxide packed, but if it is exposed to heat, it hardens in the form of blocks. How can we prevent this agglomeration and keep the mixture...
21 June 2023 6,941 1 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
Bonjour, j'ai une question si quelqu'un a une réponse. J'ai une colonne Uptisphere Strategy 100 Å Bonding HILIC-HIT avec une taille de particules de 3 µm et une longueur de 100 mm. Je n'arrive pas...
24 June 2024 7,201 1 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
how avoid forming lumps in disolving Laemnili buffer 5x? due to presence of undissolved bromoclesol blue and thick glycerol. Please tell me how to resolved this issue.
12 June 2024 10,034 3 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...
09 June 2024 6,479 2 View