Hadil Ossama Elsayed

3 Questions 3 Answers 0 Followers

Questions related from Hadil Ossama Elsayed

How long can I keep bacterial glycerol stock in -20 freezer?

I need to prepare glycerol stock for bacteria and the only available option is to keep at a -20 freezer.

25 February 2019 9,506 1 View

How do I improve band visualization of PCR?

I am testing ISSR primers on plant DNA and the bands I get are very faint. I am adding 5 microliters to the wells from a 25 microliter PCR product, I have tried increasing the volume in the well...

10 February 2019 1,564 6 View

What is a good procedure for revival of bacteria from a -80 Glycerol stock?

I have an 8 month old Glycerol stock of agrobacterium stored at -80, I have tried culturing the bacteria on LB agar and incubating overnight at 37c but there was no growth. any tips?

27 June 2018 4,117 4 View