I've been trying to find information about how long colchicine can remain as an effective inducer of polyploidy in plants while suspended in an aqueous solution containing less than 5% colchicine.
I've found information concerning the safe storage of powered colchicine, and that it reacts with light, but nothing about this in particular.
On the Cayman Chemical product information sheet it says that when stored properly Colchicine (In the powdered form) can remain stable for up to 2 years. They also say "We do not recommend storing the aqueous solution for more than one days".
If anyone could point me towards some research on the long-term viability of colchicine in an aqueous solution, or has any personal experience in trying to use and reuse colchicine I'd appreciate it.
Thanks!
Maximum you can store in fridge for 2 months. But to reduce any precipitate formation you can add 10-15%DMSO in DM water. Also DMSO might interact with plant growth in tissue culture. Few reports have been published on this
I have used colchicine extensively. Colchicine works best when you use freshly made solution. I would not recommend use of DMSO as it can cause albino problems in some plants. Use it fresh.
To my knowledge colchicine in aqeous state is stored at -20 - -40 degree C temp ( around 2 months) in dark conditions since it causes photodegradation. It gets degraded to Octahydrodemethoxydesoxydesacetamidocolchicine. Even i use aqeous colchicine standard. So every time you take out the standard from frozen condition to RT It gets degraded to some extent. so best way is to make small aliquotes.
It should be kept in powder form and dissolved in Distilled water when needed.
Thanks to all, I was keeping my colchicine stock solution up to 1 month. I think it affected my doubling frequency of chromosomes in microspores.
Appreciate everyone.
Is there a chemical that neutralizes colchicine? (Ref: Karla Y. Leyva-Madrigal) Like 10% Sodium Thiosulphate (3:1) to neutralize EMS.
Is it ok to use colchicine treatments while it is in polystyrene containers, such as a polystyrene cell culture container or does it have to be used in glass only?
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