Some researchers publicated the resultys that AAV can stably express target gene for more than >2 years, is this a common phenomenon?
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
18 February 2021 9,188 3 View
There are two plates with horizontal center crack, one with crack length of a with the other with a+da. The bottoms are fixed while the tops are under the same displacement load. For intuition,...
11 February 2021 9,543 2 View
Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...
24 January 2021 9,912 5 View
I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).
14 January 2021 3,805 2 View
I just developed a new theory on electromagnetic and plan to publish in a Journal. The findings are purely through strict mathematical derivation without any disputable assumptions and also...
13 January 2021 1,558 5 View
Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...
13 January 2021 8,336 4 View
I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...
31 December 2020 8,838 3 View
Hi, I use YASARA (version 17.8.15) on windows 10 and recently I am trying to install FoldX plugin on YASARA. Here is what I did: 1. Download YASARA plugin for windows (and the executable and...
24 December 2020 536 3 View
I am learning the knowledge about barge pier collision these days, and have a problem that the impact force of barge pier collision suddenly increase to the peak value in ls-dyna. But the...
05 December 2020 9,978 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?
02 March 2021 557 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...
28 February 2021 2,723 4 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View