What are the different way to give psychological (mental) stress in human population studies ?

01 February 2015 1,586 4 View

How to measure total body reaction time in human ?

I wan to measure total body reaction time in human

01 February 2015 394 0 View

Is there any possibility to differ auditory and visual reaction in different geographical region of same country ?

31 December 2014 7,464 3 View

Is it necessary to do GC-MS study for using crude extract to treat animals?

09 October 2014 2,834 8 View

What is the importance of ( df and F ) in one way ANNOVA analysis?

09 October 2014 2,971 4 View

What is the best method to isolate specific compound from plant extract?

09 October 2014 3,927 6 View

Can any one help me get a protocol for giemsa staining for bone marrow of wistar albino rats?

I want to do giemsa staining for bone marrow cells.

09 October 2014 8,393 5 View

Can anyone help me to get a protocol for cresyl fast violet staining for neuronal tissue?

I want to do cresyl fast violet staining for brain of wistar albino rats.

09 October 2014 550 1 View

What is the criteria for fixed dosing in vertebrates for any plant extract studies?

I want to know what the criteria should be to monitor the fixed dose for plant extracts on vertebrates (fishes).

08 September 2014 9,849 1 View

Can I use a zebra fish model to find out the brain antioxidant activity of aqueous extract of some fruit seed?

08 September 2014 8,225 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View