How large DNA fragment can be cloned in pUC19?

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Shah Faruque

Up to ~5kb may be okay. The smaller the better, and as you try to clone larger fragments, the efficiency decreases.

Tausif Alam

You would be fine with 3kb insert in any of the pUC series plasmids.

Eric van der Graaff

With cloning vectors, including pUC series, you can easily go up to 7kb insert. You still will get enough colonies with chemical competent cells (RbCl2).