Hello everyone, I am confused a little bit about the Lewis Lung Cancer Cells (LLC). In some papers their morphology seems elongated like adherent cells, however a few information mentions that they are rounded and loosely adherent. I try to seed them 3 times and each time has different morphology. In the first, they all adherented and had elongated morphology. In the second time, they were all rounded and in the third time a few of them elongated and majority of them are rounded. And in the second and third try, the medium did not change the colour so much. In the second try, their number got decreased with time. Now I try to continue with the third try and I will add their morphology.

If there is anyone who works with LLC cells, may I have their opinion and the suggestions about these issues?

Thank you so much.