Merve Çulha

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Questions related from Merve Çulha

May I have any suggestion for housekeeping genes to process RT-PCR in muscle?

Hello everyone, I work with C57BL/6 mice and administer exercise to them to process PCR in muscle tissue. However, I have not yet found a suitable housekeeping gene. We tried GAPDH, HPRT1, 36B4,...

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How is the process of Lewis Lung Cancer (LLC) cells morphology alteration?

Hello everyone, I am confused a little bit about the Lewis Lung Cancer Cells (LLC). In some papers their morphology seems elongated like adherent cells, however a few information mentions that...

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How can I design the miRNA primers for RT PCR?

Hello everyone, I would like to ask a question to manage my experiment. To decrease the budget of my experiment, I have been searching for designing miRNA primers for RT PCR. I could find just a...

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Do you have a protocol about purification preadipocytes from fibroblasts?

I want to try primary culture on preadipocytes for my thesis. I will use human adipose tissue for primary culture. I found a protocol for seperating preadipocytes from adipocytes but I don't know...

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