How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

High pass filter on EMG after Bandpass(Boonstra2012)?

Hi all, I am currently trying to carry out an EMG coherence analysis, prior to this I have been reading some papers. I can understand the Bandpass implication from range A Hz to B Hz, but from...

25 November 2020 7,461 1 View

How can I design resin column for test?

I'm interested in the research field of ion exchange resin. I want design about resin column with acryl for test. How do you usually design the diameter and length of small columns for test? I...

13 October 2020 3,443 2 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

How do I change to make an insoluble recombinant protein to a soluble protein?

How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...

05 October 2020 1,840 2 View

Can XRD determine inorganic material composition?

From my understanding, XRD is used to determine phase identification of a crystalline material. Is it possible to use XRD to determine the composition of a certain material? I think XRF or EDS is...

15 June 2020 9,211 8 View

What are the steps before perform Normality Test?

Hi all, I m a Master Students. I need to perform Normality Test in my study. I have 5 independents variables and 1 dependent variable. Each independents have 6-8 questions and dependent variable...

14 May 2020 9,015 3 View

How to fit the Gaussian curve based on the moment of power spectrum?

I want to make a spectral correction curve of my light source to resemble the Gaussian spectrum. This literature said, ".. The dashed curve is a Gaussian fit with a center wavelength of 833 nm...

20 January 2020 8,950 2 View

Sodium chloride solution saturated vapor pressure at sub zero temperature?

Can anyone suggest where to find the data or any equations related to the salt solution vapor pressure at sub-zero temperature? Many thanks!

24 December 2019 7,741 2 View

Can aequorin being stably transfected in cell line?

We are trying to transfected the aequorin plasmid into neurons, how can aequorin being stably transfected in cell line?

09 September 2019 1,386 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View