How is the graph plotted for congeneric and conspecific K2P divergence?

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Anyone using CyDisCo sytem for expression of proteins in E. coli?

I am currently working on several proteins with at least 4 disulfide bonds. I am isolating inclusion bodies and refolding the proteins. I would like to try expression of the same using CyDisCo...

03 April 2019 560 4 View

What apoptotis pathway are to be targeted in mosquito larva?

We are working on larvicidal plant extract and would like to study the effect of larvicides on mosquito larvae. What genes are to be targeted for pPCR studies?

09 October 2017 601 2 View

Does anyone have experience with periplasmic expression of a 7.6 kDa cysteineless protein?

We have cloned a gene into pET20b. The clone was sequenced and the gene is in the correct frame. However, we are having difficulties in expression of the protein. We do not have antibodies to...

02 March 2014 6,752 3 View

Can someone help with a problem in DNA isolation?

We are trying to isolate DNA from liver tissue of snake preserved in alcohol/RNAlater using phenol:chloroform method. However, we are not getting good quality DNA. There is smearing or trailing of...

11 December 2013 6,013 11 View

Can anyone suggest solutions regarding a problem with high current when running tris-tricine SDS-PAGE gel?

I use 18% tris-tricine SDS-PAGE gel (ethylene glycol added) for separating protein samples. I use 0.1M Tris, 0.1M tricine, 0.4% SDS buffer for running the gel. I run the gel at constant voltage of...

10 November 2013 2,889 1 View

How to infer genetic or nucleotide diversity between different species?

We can easily calculate genetic/nucleotide diversity using softwares. For example, DnaSP. But, what does the values signify (Pi, Theta, Eta, S, K). How to interpret the result? Say Pi=0.0945 &...

07 August 2013 2,899 0 View

How do we identify a bacteria on a species or subspecies level?

It is seen that generally people sequence 16S rDNA for the identification of a bacterium. However, it has low phylogenetic power at the species level (Janda and Abbott, 2007) and cannot be used to...

02 March 2013 4,912 92 View

Does anyone have cloning vector with Sfi I restriction site?

We need a cloning vector with Sfi I restriction site. We would be grateful if anyone can provide the same to us.

11 December 2012 6,884 8 View

Why is sound not working in my Ubuntu system?

I have installed Biolinux 6 based on ubuntu 10.04. However, sound is not working even after installation of alsa utilis, alsamixergui and adding of "options snd-hda-intel probe_mask=1 model=hp"...

10 November 2012 5,170 6 View

Has anyone tried to clone cDNA into TA vector??

I have synthesized cDNA in my lab using Clontech SMART cDNA synthesis kit. I am trying to insert the cDNA into TA vector. However, this didn't work. Could anyone suggest or comment on the idea of...

09 October 2012 9,449 11 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View