How is the graph plotted for congeneric and conspecific K2P divergence?

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Anyone using CyDisCo sytem for expression of proteins in E. coli?

I am currently working on several proteins with at least 4 disulfide bonds. I am isolating inclusion bodies and refolding the proteins. I would like to try expression of the same using CyDisCo...

03 April 2019 453 4 View

What apoptotis pathway are to be targeted in mosquito larva?

We are working on larvicidal plant extract and would like to study the effect of larvicides on mosquito larvae. What genes are to be targeted for pPCR studies?

09 October 2017 506 2 View

Does anyone have experience with periplasmic expression of a 7.6 kDa cysteineless protein?

We have cloned a gene into pET20b. The clone was sequenced and the gene is in the correct frame. However, we are having difficulties in expression of the protein. We do not have antibodies to...

02 March 2014 6,674 3 View

Can someone help with a problem in DNA isolation?

We are trying to isolate DNA from liver tissue of snake preserved in alcohol/RNAlater using phenol:chloroform method. However, we are not getting good quality DNA. There is smearing or trailing of...

11 December 2013 5,929 11 View

Can anyone suggest solutions regarding a problem with high current when running tris-tricine SDS-PAGE gel?

I use 18% tris-tricine SDS-PAGE gel (ethylene glycol added) for separating protein samples. I use 0.1M Tris, 0.1M tricine, 0.4% SDS buffer for running the gel. I run the gel at constant voltage of...

10 November 2013 2,801 1 View

How to infer genetic or nucleotide diversity between different species?

We can easily calculate genetic/nucleotide diversity using softwares. For example, DnaSP. But, what does the values signify (Pi, Theta, Eta, S, K). How to interpret the result? Say Pi=0.0945 &...

07 August 2013 2,795 0 View

How do we identify a bacteria on a species or subspecies level?

It is seen that generally people sequence 16S rDNA for the identification of a bacterium. However, it has low phylogenetic power at the species level (Janda and Abbott, 2007) and cannot be used to...

02 March 2013 4,811 92 View

Does anyone have cloning vector with Sfi I restriction site?

We need a cloning vector with Sfi I restriction site. We would be grateful if anyone can provide the same to us.

11 December 2012 6,806 8 View

Why is sound not working in my Ubuntu system?

I have installed Biolinux 6 based on ubuntu 10.04. However, sound is not working even after installation of alsa utilis, alsamixergui and adding of "options snd-hda-intel probe_mask=1 model=hp"...

10 November 2012 5,088 6 View

Has anyone tried to clone cDNA into TA vector??

I have synthesized cDNA in my lab using Clontech SMART cDNA synthesis kit. I am trying to insert the cDNA into TA vector. However, this didn't work. Could anyone suggest or comment on the idea of...

09 October 2012 9,362 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View