What apoptotis pathway are to be targeted in mosquito larva?

More Rajeev Kungur Brahma's questions See All

Anyone using CyDisCo sytem for expression of proteins in E. coli?

I am currently working on several proteins with at least 4 disulfide bonds. I am isolating inclusion bodies and refolding the proteins. I would like to try expression of the same using CyDisCo...

03 April 2019 453 4 View

How is the graph plotted for congeneric and conspecific K2P divergence?

The image of the Congeneric and conspecific K2P divergence for 101 sequences has been given from a paper (link attached). Please explain.

03 April 2016 7,615 2 View

Does anyone have experience with periplasmic expression of a 7.6 kDa cysteineless protein?

We have cloned a gene into pET20b. The clone was sequenced and the gene is in the correct frame. However, we are having difficulties in expression of the protein. We do not have antibodies to...

02 March 2014 6,674 3 View

Can someone help with a problem in DNA isolation?

We are trying to isolate DNA from liver tissue of snake preserved in alcohol/RNAlater using phenol:chloroform method. However, we are not getting good quality DNA. There is smearing or trailing of...

11 December 2013 5,929 11 View

Can anyone suggest solutions regarding a problem with high current when running tris-tricine SDS-PAGE gel?

I use 18% tris-tricine SDS-PAGE gel (ethylene glycol added) for separating protein samples. I use 0.1M Tris, 0.1M tricine, 0.4% SDS buffer for running the gel. I run the gel at constant voltage of...

10 November 2013 2,801 1 View

How to infer genetic or nucleotide diversity between different species?

We can easily calculate genetic/nucleotide diversity using softwares. For example, DnaSP. But, what does the values signify (Pi, Theta, Eta, S, K). How to interpret the result? Say Pi=0.0945 &...

07 August 2013 2,795 0 View

How do we identify a bacteria on a species or subspecies level?

It is seen that generally people sequence 16S rDNA for the identification of a bacterium. However, it has low phylogenetic power at the species level (Janda and Abbott, 2007) and cannot be used to...

02 March 2013 4,811 92 View

Does anyone have cloning vector with Sfi I restriction site?

We need a cloning vector with Sfi I restriction site. We would be grateful if anyone can provide the same to us.

11 December 2012 6,806 8 View

Why is sound not working in my Ubuntu system?

I have installed Biolinux 6 based on ubuntu 10.04. However, sound is not working even after installation of alsa utilis, alsamixergui and adding of "options snd-hda-intel probe_mask=1 model=hp"...

10 November 2012 5,088 6 View

Has anyone tried to clone cDNA into TA vector??

I have synthesized cDNA in my lab using Clontech SMART cDNA synthesis kit. I am trying to insert the cDNA into TA vector. However, this didn't work. Could anyone suggest or comment on the idea of...

09 October 2012 9,362 11 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Sang Ho Lee

Hello, Rajeev.

You could list up of pro-survival and pro-apoptotic genes including Bcl2 and Bax, homologs etc, and stress genes such as iNOS, HSP70, COX2 homologs in invertebrates. It would be more appropriate if you have Western blot data than RT-PCR.

Best wishes.

Rajeev Kungur Brahma

Thank you Dr Lee for your input.