12 Questions 26 Answers 0 Followers
Questions related from Rajeev Kungur Brahma
I am currently working on several proteins with at least 4 disulfide bonds. I am isolating inclusion bodies and refolding the proteins. I would like to try expression of the same using CyDisCo...
04 April 2019 526 4 View
We are working on larvicidal plant extract and would like to study the effect of larvicides on mosquito larvae. What genes are to be targeted for pPCR studies?
10 October 2017 561 2 View
The image of the Congeneric and conspecific K2P divergence for 101 sequences has been given from a paper (link attached). Please explain.
04 April 2016 7,668 2 View
We have cloned a gene into pET20b. The clone was sequenced and the gene is in the correct frame. However, we are having difficulties in expression of the protein. We do not have antibodies to...
03 March 2014 6,725 3 View
We are trying to isolate DNA from liver tissue of snake preserved in alcohol/RNAlater using phenol:chloroform method. However, we are not getting good quality DNA. There is smearing or trailing of...
12 December 2013 5,984 11 View
I use 18% tris-tricine SDS-PAGE gel (ethylene glycol added) for separating protein samples. I use 0.1M Tris, 0.1M tricine, 0.4% SDS buffer for running the gel. I run the gel at constant voltage of...
11 November 2013 2,858 1 View
We can easily calculate genetic/nucleotide diversity using softwares. For example, DnaSP. But, what does the values signify (Pi, Theta, Eta, S, K). How to interpret the result? Say Pi=0.0945 &...
08 August 2013 2,856 0 View
It is seen that generally people sequence 16S rDNA for the identification of a bacterium. However, it has low phylogenetic power at the species level (Janda and Abbott, 2007) and cannot be used to...
03 March 2013 4,870 92 View
We need a cloning vector with Sfi I restriction site. We would be grateful if anyone can provide the same to us.
12 December 2012 6,856 8 View
I have installed Biolinux 6 based on ubuntu 10.04. However, sound is not working even after installation of alsa utilis, alsamixergui and adding of "options snd-hda-intel probe_mask=1 model=hp"...
11 November 2012 5,139 6 View
I have synthesized cDNA in my lab using Clontech SMART cDNA synthesis kit. I am trying to insert the cDNA into TA vector. However, this didn't work. Could anyone suggest or comment on the idea of...
10 October 2012 9,417 11 View
I am trying to separate venom proteins on tricine SDS-PAGE, but separation is not good. I have tried with nature protocols and current protocols in mol bio methods, and have tried 20% and 16.5%...
06 June 2012 5,240 0 View
