ELISA micro-plate reader emulator "Digital-OD"

06 July 2017 7,421 3 View

How can i download seq from NCBI FTP server?

I want to download a seq from the NCBI FTP server,I tried to download with ftp clinet like filezella and sftp but both didnt recognize the assamply folder and failed to download any files or...

01 February 2016 9,371 0 View

What is the minimum requirement for local Galaxy instance for single user?

i'm planning to install my own Galaxy instance on my laptop which is Core I3 with 8GB ram and 15GB assigned as SWAP. is this will be fast or not compared to the main server or i have to use cloud?

10 November 2015 704 0 View

Is pyrogen free water be nuclease (DNAse/RNAse ) free?

05 June 2015 4,002 0 View

how does DNA sequencing device determine the F and R primers in PCR product?

i have two files from the DNA sequencer one for the foreword strand and the second for the reverse strand how the device separate them and does it can recognize the direction of the primer

03 April 2014 8,039 5 View

What is the perfect length of 16s rRNA gene suitable for an accurate species identification of unknown isolate?

Molecule identification of unknown is very helping tool using 16s rRNA gene analysis. In some cases the output file only contains about readable 600 base pairs. Is that enough to identify the...

02 March 2014 6,748 16 View

Can the monoclonal antibodies (Mab) reaction with Seabream (Sparus aurata) be used to detect immunoglobulin M (IgM) Argyrosomus regius?

I want to use this antibody to detect the immunoglobulin M (IgM) of Argyrosomus regius.

02 March 2014 9,196 2 View

Is there any other test that could replace ONPG test in biochemical tests of unknowns?

ONPG is one of the important biochemical tests for biochemical identification of unknowns. Which test can replace it?

01 February 2014 3,383 9 View

No title

02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View