Our aim was to compare the differential activity (if any) for our protein of interest that is involved in lipid metabolism (can be quantified using PC/PE ratio, already shown in mice and human serum samples). Therefore, we gathered lipidomic data from our wild type vs mutant organism, using high-resolution mass spectrometry system (HRMS) coupled with liquid chromatography. The lipidomic data exhaustively quantified a lot of different species of lipids including 100's of different species/ subclasses of PC and PE. Specific PC or PE species co-relation with our protein of interest was never shown. So I am trying to understand:

- If there are strategies to normalize these species to give a cumulative PE or PC values that could be used to plot the data?

other suggestions?

Thanking in advance