Pankaj Thapa

6 Questions 13 Answers 0 Followers

Questions related from Pankaj Thapa

How is Phosphatidylcholine (PC)- Phosphatidylethanolamine (PE) ratio calculated? #lipid #analysis #worm #c.elegans #massspec ?

Our aim was to compare the differential activity (if any) for our protein of interest that is involved in lipid metabolism (can be quantified using PC/PE ratio, already shown in mice and human...

24 March 2022 6,722 0 View

How to solve or make 2D construction maps from negative staining images?

I have managed to get good images from negative staining grids of protein complexes (120 to 230 kDa) which seem homogenous and consistent with regards to the radii as per their molecular weights...

24 October 2018 4,616 2 View

How to model a dimer from a monomer pdb?

Hi, I wish to generate a dimer model of protein X, which I modeled using modeling tool (Swiss model using crystal structure of mouse, has homology ~96%, was solved as a dimer). The modeled...

10 August 2018 5,512 11 View

What is the acceptable amount of sarkosyl for protein purification?

I am trying to purify a protein involved in DNA repair and is a part of supra-molecular complex. This protein was over-expressed using pET28a construct in BL21 2DE3 cells but its induction was...

12 January 2018 1,158 3 View

Need suggestion for suites or tools for building the model of protein derived from X ray diffration data

I have managed to solve the structure of a protein for which X ray diffraction data was collected and using a template structure, which was only missing 13 amino acids from the N terminal side,...

25 July 2017 8,310 3 View

List of Breast Cancer associated proteins?

I wish to carry out an exhaustive study of all the proteins which are involved/associated in/with breast cancer (associated directly or indirectly both, with regards to its progression, increase...

16 October 2016 1,754 4 View