Is there a good protocol to label membrane using FM 1-43?
I wish to study membrane dynamics. However, I am getting unwanted fluorescence in the solution while doing TIRF.
Hi Deepthi,
You may want to have a look at this paper. It discusses the labeling protocols and constraints related to FM 1-43 labeling in endothelial cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1630457/
You might also want to consider using GFP tagged plasma membrane localizing probes(plasmid constructs) such as CD36 , cadherin or another membrane marker for epithelial cells to study membrane dynamics
http://www.leica-microsystems.com/science-lab/tirf-microscopy-of-the-apical-membrane-of-polarized-epithelial-cells/
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