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Questions related from Deepthi Thomas
Is there a good protocol to label membrane using FM 1-43? I wish to study membrane dynamics. However, I am getting unwanted fluorescence in the solution while doing TIRF.
29 September 2016 8,144 1 View
Even with as low as 0.5 microlitre volume of FM-dye, laser power as low as 10%, penetration depth of 25nm, my cell sample illuminates like it does epifluorescence. I am using Olympus CellLineTIRF 4.
26 August 2016 5,632 1 View
