How is chimera detection for 18S rDNA sequence carried out?

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How long can PBMCs be stored at -70°C with good quality?

Can storing PBMCs at -70°C for a long time affect the population of PBMCs?

15 January 2024 8,010 3 View

Method of measuring sulfur for molybdenum oxide?

What is the method of measuring sulfur for minerals such as metal oxide or molybdenum oxide through Leco furnace (or similar furnaces) at different temperatures, times and weights after leaching...

11 November 2023 3,345 2 View

What is the best cytotoxicity test for car-nk cell evaluation? (with protocol)?

cytotoxicity test

30 July 2023 1,006 1 View

Nursing curriculum and educational system in canada and emarat countries?

i like to know some thing about nursing system in these country something like their salary and other things

06 December 2022 7,604 3 View

How to simulate turbines via EPANET toolkit in Python?

Hi researchers, I utilised epanet toolkit in Jupiter to model a micro pumped storage system. To model the turbine I substitute it with GPV. However, I faced an error when I was about assigning the...

08 November 2022 2,639 3 View

How can i find the rpm of my wind turbine in non rated speed?

my turbine is a variable speed wind turbine.

18 May 2022 7,250 4 View

Hi everyone. Does high concentrations of Tris-HCL buffer ( 2-3 mM Tris in 25 μl real time PCR reaction ) interfere with PCR reaction?

I have dissolved my primers in TE buffer 10X, which in the final PCR reaction will have an EDTA concentration of 0.02 mM and a Tris-HCL concentration of 2-3 mM. Will this interfere with the tag...

14 February 2022 6,667 3 View

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

07 December 2021 9,234 7 View

How can I measure electrical conductivity (EC) in mucilage seeds?

I want to measure EC in mucilage seeds. I do not want the seed coat to be damaged. So, how can I measure EC ? Is there another way to replace the electrical conduction method for mucilage seeds?

30 November 2021 6,539 5 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

21 November 2021 9,368 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View