I used MP-5 medium and cetrimide agar for pectinolytic activity. for this firstly, I prepared MP5 medium and spotted on plates from cultures, after incubation added %1 cetrimide agar. Finally I didn't see clear zone arround the microorganisms. I don't know to solve this problem. Can you help me?
1% cetrimide is apparently the disclosing agent (http://www.sciencedirect.com/science/article/pii/S0944501306000966).
Cetrimide agar apparently doesn't serve that function - it has less cetrimide (0,3%) and that lesser amount is spatially limited by agar. suggest you follow the published procedure with 1% solution.
I used a 7.5% copper sulphate solution, for 1 - 2 hours, to visualise Pel activity when I worked with Pectobacterium - don't know if it is specific to the type of medium I used, or could be applied to other media.
I think copper acetate (saturated) would also work.
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