Spectral matching is the obviously required for efficient FRET. But for FRET between Quantum dots (donor) and dye (acceptor), what is the role of surface functionalization. For example if one is amine functionalized and other is carboxy functionalized or both are carboxy functionalized, what would be the possibility of FRET, acceptor emission enhancment and acceptor emission quenching?
Thanks for your answers.
Beside spectral overlap, spatial connection between the donor and acceptor is also an important factor for efficient FRET since the efficiency depends upon the distance between donor and acceptor. In many FRET is inefficient if the donor and acceptor are not close enough. In your case, the amine-carboxylic acid pair could help. But, you have to be careful about the emission intensity of your dye since amine can also be a source of electron, meaning that, photoinduced electron transfer can also be another possibility depending upon the redox potential matching.
Hi Fahad, I think you should examine the fluorescence properties of individual surface bound fluorophores before doing FRET.
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