I'm MS student and its start of Research. Can anyone please guide me how I can make concentrations for DPPH?
Dear Aqsa Hayyat
I hope you meant DPPH radical scavenging activity. DPPH free radical scavenging activity(%) = {(C−D) − (A−B)}∕(C−D) × 100
here C is the absorbance of DPPH + solvent, D is the absorbance of solvent + methanol, A is the absorbance of sample or standard + DPPH, and B is the absorbance of sample or standard + methanol
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weigh 4mg of DPPH and added into methanol.
The free radical scavenging activity of the extracts, based on the scavenging activity of the stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical, was determined by slightly modified method described by Brand-Williams et al. [13]. Different concentration of plant extract were added to 3 mL of a 0.004% methanolic solution of DPPH and incubated for 15 minutes at room temperature. Absorbance was recorded at 517 nm by using spectrophotometer (Thermo Scientific UV1)
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DPPH (2,2-diphenyl-1-picrylhydrazyl) is a widely used stable free radical that can be used to measure the antioxidant capacity of a substance. To create DPPH solutions of different concentrations, you will need to start with a stock solution of DPPH and dilute it to the desired concentration.
Here are the steps to prepare a DPPH solution:
To make solutions of different concentrations, you can use the stock solution and dilute it with the solvent of your choice. For example, to prepare a 0.01 mM solution of DPPH, you can take 1 mL of the stock solution and dilute it with 9 mL of solvent. This will give you a 0.01 mM DPPH solution.
The concentrations of DPPH that you use will depend on the specific experiment you are conducting. It is important to note that DPPH solutions are light-sensitive and should be stored in a dark container at room temperature.
Muhammad Azam
May be, you are wrong, to prepare 0.1 mM stock solution of DPPH, we need to add 3.9432 mg of DPPH in 100 ml.
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