I am relatively new at bioinformatics, and I am trying to model an enzyme for docking analysis. I have used modeller, but the template of my enzyme does not include de metal atoms that I know are needed for the activity, and are for that reason essential before the docking analysis.
I guess that I need to modify something in the PDB file, and then modelling and refining, but I am not sure of how to do it. If anybody has done something similar, I would like to know your experience.
Thanks a lot!