01 January 2013 1 717 Report

I have also the Orfs.

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Why phage head look like empty after TEM negative staining- can anyone give the answer?

I am using 1% Ga cacodylate-filtered buffer for quick hard fix of my phage sample to grid then perform negative staining by 2% uranyl acetate. But the phage picture look like empty. Does it mean...

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GST-fusion protein elution-can anyone help?

I have 9 recombinant GST-tagged proteins. Maximum proteins formed inclusion bodies. I use one kit for solubilization and renaturation. It works well but problem is in elution. Proteins not elute...

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Can you recommend a good GST inclusion body solubilization and renaturation kit?

I used GST gene fusion system (pGEX-6P-1) for protein expression and found all of my recombinant proteins deposited in the form of inclusion bodies. I changed the conditions (IPTG conc, induction...

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