Can you recommend a good GST inclusion body solubilization and renaturation kit?

Shakhinur Islam Mondal @Shakhinur_Islam_Mondal

08 August 2014 0 5K Report

I used GST gene fusion system (pGEX-6P-1) for protein expression and found all of my recombinant proteins deposited in the form of inclusion bodies. I changed the conditions (IPTG conc, induction time, tem) but no advantages, all remain in inclusion bodies. So I would like to solubilize and refold my proteins. Is there any good commercial kit available for this purpose?

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