How good is DNAzol in extraction of fungal DNA?

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I would like to assign taxon IDs to ITS amplicon sequences obtained using ITS 1 and ITS 4 primers. What would be the best program to use to get the nearest neighbors from the genebank?

10 November 2019 6,238 1 View

What would be the best way to start treatment of highly invasive cancer?

Chemotherapy or radiotherapy and what would be the advantage of the choice? One doctor advised to start with chemotherapy and another advised to start with radiotherapy.

09 October 2013 5,212 14 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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How to increase a model accuracy ?

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Is there a powerful system for the security of the systems distributed on IoT systems?

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How Relativistic effects is one reason for lanthanide contraction?

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01 March 2021 2,272 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Which cell line should we use to perform biological dosimetry experiments?

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Isolates of Hymenoscyphus fraxineus?

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How to remove the residual PDMS on the silicon mold?

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For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

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Yashwant Chavan

If you extract DNA using DNAzol, there are chances of higher amount of proteins coming in your final DNA preparation as this method demands much careful separation of phases. You may instead try any spin-column protocol.

Christina Burns

We are studying a fungal phytopathogen and have seen good results using a spin column protocol (MPBio, I believe) to extract DNA from infected tree root samples.