How exactly we can separate mitochondrial protein from cytoplasmic protein of cell through centrifugation?

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Adam B Shapiro

Here is a protocol for the preparation of mitochondria from cell culture. The supernatant contains the cytoplasmic proteins.

https://www.abcam.com/ps/pdf/protocols/mitochondrial%20purification%20protocol.pdf

Lina A. Hassan

I think this scheme of this article will help be you

Article A simple protocol for the subcellular fractionation of skele...

Archana Samal

It is really helpful, Thank you very much for your answer.