Not all PolII transcripts (capped, tailed, etc)are turned over at the same rate. How is Xrn1-mediated turnover regulated? What regulates Xrn1 degradation of alternate transcripts from the same gene?
This paper explains the biochemistry of Xrn1: http://www.ncbi.nlm.nih.gov/pubmed/11586897
Xrn1 has been shown to bind with Dcp-1, a decapping activator linking it to the decapping enzyme Dcp2 to couple the two processes: http://www.ncbi.nlm.nih.gov/pubmed/23142987
As far as regulation goes I think that would be more on the regulation of the decapping reaction and since some mRNAs are recapped in the cytoplasm to protect them from Xrn1 degradation the regulation would be at multiple levels (deadenylation, decapping, etc). Our lab is investigating what makes mRNAs targets for recapping and protection from Xrn1 so stay tuned for future publications.
This paper explains the biochemistry of Xrn1: http://www.ncbi.nlm.nih.gov/pubmed/11586897
Xrn1 has been shown to bind with Dcp-1, a decapping activator linking it to the decapping enzyme Dcp2 to couple the two processes: http://www.ncbi.nlm.nih.gov/pubmed/23142987
As far as regulation goes I think that would be more on the regulation of the decapping reaction and since some mRNAs are recapped in the cytoplasm to protect them from Xrn1 degradation the regulation would be at multiple levels (deadenylation, decapping, etc). Our lab is investigating what makes mRNAs targets for recapping and protection from Xrn1 so stay tuned for future publications.
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