Agniva Saha

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Questions related from Agniva Saha

How can I make drop out media plates containing Kanamycin or Nat drugs for yeast ?

I found out that Nat or Kan selection does not work on drop out plates. The ammonium sulfate interferes with drug selection. I have read that using proline or glycine instead of ammonium sulfate...

02 February 2019 2,364 3 View

How can one determine if a cellular RNA is 5'capped or not?

I am studying a novel transcript in yeast and would like to know if my RNA has a 5'7mG cap. I have tried treating RNA with a commercial cap-dependent RNase (Terminator exonuclease/Xrn1) but it...

09 September 2014 2,941 4 View

How does Xrn1 select RNAs for degradation? In other words, what characteristics of RNA make it a Xrn1 target?

Not all PolII transcripts (capped, tailed, etc)are turned over at the same rate. How is Xrn1-mediated turnover regulated? What regulates Xrn1 degradation of alternate transcripts from the same gene?

12 December 2013 8,072 2 View

How can mutations outside a transcription unit, that don't alter synthesis or stability of the RNA, affect its function?

Can mutations that map upstream of a transcript (promoter, etc) lead to loss of function of the transcript, without altering expression or stability of the RNA? It is a non-coding RNA transcribed...

09 September 2013 8,971 10 View

Does the budding yeast Saccharomyces cerevisiae make RNAs smaller than snoRNAs and tRNAs?

Budding yeast S. cerevisiae lacks genes for RNAi. They do not make siRNAs. What is the smallest size class of RNA produced by this organism?

09 September 2013 7,139 4 View