How does the overexploitation of granular aquifers trigger desertification processes?

Rafael Lima dos Santos Santos @Rafael-Lima-Dos-Santos-Santos

05 April 2024 0 4K Report

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Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,807 3 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

12 May 2024 1,045 4 View

How can I download my publications list and citations list...?

How can I ? need to renew my cv and need list of my publications and citations

07 May 2024 5,554 4 View

If thermodynamics obtains a positive Gibbs energy in the adsorption process with negative enthalpy and entropy, how can it be justified?

ΔG = 12.77 ΔH = -37,92 ΔS = -162,40

07 May 2024 4,942 3 View

How does YMB medium looks like after a few days of bacteria inoculation?

Hello, everyone I've recentely isolated bacteria from pigeon pea (Cajanus cajan) nodules on YMA medium and then transferred some colonies to YMB (Yeast Mannitol Broth) medium. This is the first...

01 May 2024 3,296 4 View

What are the key methods and indicators used in assessing the biodiversity of river ecosystems, and how do these methods account for variations ?

Biodiversity assessment of river ecosystems is crucial for understanding the health and stability of these environments. This question aims to explore the various techniques employed to evaluate...

07 August 2024 4,290 3 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

Focus on water use optimization or water resource optimization which one is effective way toward sustainability?

Water resources are limited and have been significantly reduced by pollution due to urbanization in recent decades. Therefore, water resource optimization only highlights the ways these resources...

27 July 2024 9,303 1 View

What is the optimal financial policy to be followed at the present time?

I believe that we must resort to a more conservative financial policy, that is, less expenditure on the current side than it is at the present time, in addition to high coordination with monetary...

24 July 2024 7,262 2 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View