Dear Vishal,

Potassium dichromate in 2% solution prevent from fungi development. Additionally, this solution provide good oxygene conditions needed for coccidia sporulation.

Best wishes 

JK

Thank you very much, Jerzy  Kowal .

As has been said, it is related to oxygenation and suppression of growth of some microorganisms.

         Potassium dichromate with concentration of 2 to 2.5% (not more or less), helps killing and preventing the growth of ciliated and non-ciliated protozoa (micro-flora) and some bacteria. Both micro-organisms contribute in consuming oxygen and nutrients essential for the growth and development sporozoites. Moreover the yellowish coulor of dichromate can act as a stain for oocysts.

Just a comment to the sporulation with potassium dichromate. Please note that a damaged oocyst wall of Eimeria may also be susceptible to the toxic properties of the chemical. We noticed this possibility when studying the difference using the sporulation medium and not using a sporulation medium in freeze-thawing experiments. Though it depends on your study set-up this may bias some of the measurable factors, such as the oocyst count.

"Recovery and sporulation of bovine Eimeria oocysts after exposure to sub-zero temperature."

http://vetzoo.lsmuni.lt/data/vols/2014/66/pdf/lassen.pdf

Hi everyone, may I know how to prepare 2.5% potassium dichromate in a proper way. Thank you

Milli B. Nath-Chowdhury

Thank you very much for sharing this information.

Kindly consult the below-mentioned article Vishal Magadum. Potassium sorbate solution has been used as a sporulation medium.

Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution

• DOI: 10.1007/s00436-020-06792-3