In the compressed liquid state, why does the temperature remain constant even if pressure is gradually decreased? But not in the saturated state?

31 May 2020 7,801 1 View

PCR with fluorescent tag on Tecan SPARK?

My boss wants me to run PCR with a fluorescent tag. He says we can use the Tecan SPARK machine that we have for this. I am confused however. Do I run the PCR per usual with the thermocycler then...

15 March 2020 3,765 2 View

Environmental and Occupation Epidemiology Professional Development?

I am nearing the end of my first year of applied epidemiology at a local health department, I have been exposed to a diverse range of topics but throughout graduate school and my project...

05 December 2019 2,430 1 View

NOAA Hourly Precipitation Data Missing Post 2014?

Hello all, I'm trying to test out a predictive model but I'm having a very hard time trying to find hourly precipitation data. I've looked at NOAA and on the new data repository (NCDC) but I...

28 October 2019 4,757 5 View

How can I conduct multiple multilevel meta-analyses using metafor in R?

For my master's thesis, I am using meta-analytic techniques to explore the relationship between LGBTQ community connectedness and indicators of physical health. I am conducting multilevel...

13 October 2019 6,940 3 View

How do I know that I can trust the chemical analysis results of surface and ground water quality samples from a subcontractor?

I was unable to perform the sampling and analysis for 40 surface water and 20 groundwater samples, so I hired an in-country consultant firm to do it. The results that they returned were difficult...

21 October 2018 8,937 10 View

Hello, can anyone recommend which social cognitive model in health can help reduce the risk of hiv contraction ?

Hello, i am conducting a health report for a psychological assignment, this report is based on the reduction of risk in contracting hiv in a prison setting. Unfortunately after reading a fair...

26 December 2017 3,947 3 View

What is the best Havahart trap for trapping Columbian Ground Squirrels?

Small vs Medium? Normal vs Easy Set? Or are Tomahawks or XL Sherman a better fit?

07 November 2016 7,148 3 View

What resources (national organizations, museums, etc) do ESL teachers use most?

If possible please note the developmental stage (early childhood, elementary, middle school, high school) associated with each resource.

03 April 2013 1,073 2 View

Historical importance of the LGBTQ community?

Hi everyone, I'm looking for some articles that discuss the historical importance of the LGBTQ community. I'm specifically looking for theory papers that talk about why a sense of connectedness to...

01 January 1970 7,449 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View