Golla Ramanjaneyulu

15 Questions 12 Answers 0 Followers

Questions related from Golla Ramanjaneyulu

Which is the best on-line database for the construction of a phylogenetic tree for fungi?

Hello Every one, I have isolated and sequenced ITS regions for identification of fungi by Sangers method. To construct a phylogenetic tree which is the best tool available in on-line...

03 March 2015 10,027 9 View

Why does the β-Glucosidases not have the effect on the sucrose?

Any suggestions? In the literature there is a knowledge as ‘‘β-Glucosidases (EC 3.2.1.21; β-D-glucoside glucohydrolase) hydrolyze terminal, nonreducing 1,4-linked-β-glucose residues by releasing...

02 February 2015 5,919 7 View

How can I optimize culture conditions of enzyme production by using Response Surface Methodology?

Hello Every one, I have done experiments on enzyme production and optimization of production parameters like temp, pH, ...etc. Now I have to optimize the significant parameters by using Response...

01 January 2015 713 4 View

What is the best method to isolate/extract fungal genomic DNA?

What is the best method to isolate/extract fungal genomic DNA? I have tried several methods without the addition of chitinase, but I wasn't successful in this aspect. Please suggest a better...

12 December 2014 5,009 10 View

How can I prepare a smear of chemical pretreated sawdust for SEM Analysis?

Hello Every one, I am working on pretreatment of lignocellulosic biomass, I am facing a problem while preparing a smear for SEM analysis of pretreated sawdust. please let me know the smear...

12 December 2014 8,409 3 View

How can I calculate the xylose reductase and xylose dehydrogenase activities?

Hello Every One, I have taken UV spec readings (for 3 minutes) for the activity of Xylosereductase and xylose dehydrogenase by the method defined elsewhere by using NADPH/ NAD. from that...

12 December 2014 3,777 3 View

What is the role of acids in lignocellulosic biomass pretreatment?

Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...

10 October 2014 7,018 9 View

Is there any method for detection of Glu, Xylose and Xylitol by HPLC?

Hello Everyone, Please let me know, if there is any single or different methods available for detection of glucose, xylose and xylitol by HPLC equipped with C18 column and UV detector. If not...

08 August 2014 9,050 15 View

How one can analyse the biomass to quantify the reducing sugars?

Lignocellulose is a renewable biomass which is widely available in nature. It contains cellulose (glucose), hemicellulose (xylose, arabinose, mannose etc.) and lignin. How one can analyse a...

08 August 2014 5,269 2 View

How does one quantify Cellulose, Hemicellulose and Lignin?

How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?

08 August 2014 9,108 23 View

How does one convert released reduction sugars (mg/ml) to enzyme activity (IU)?

I have assayed cellulose activity, by DNS method, I got released reducing sugar in mg/ml for this. How do I calculate enzyme activity and express in IU?

07 July 2014 9,701 11 View

How to find the synthesized derivatives of natural compounds which are synthesized earlier or not?

We are planning to synthesize the new derivatives of natural compound (Ex-curcumin) has not yet synthesized elsewhere. Hence, please let me know the tools to find out all derivatives available for...

07 July 2014 4,789 3 View

How should I calculate the ethanol percentage to mg/ml?

I have 99.9% of ethanol MW is 46.07 Density is 0.789 Based on above values, how can I convert to mg's? I need 1 mg/ml for this. How much of ethanol do I have to add to the water to get 1 mg/ 1...

05 May 2014 5,138 13 View

Is it possible to detect xylitol with GC?

Is it possible to detect xylitol with Gas Chromatography (GC)? If it's possible what are the conditions for detection of xylitol with GC? We have a GC with capillary column and FID detector. What...

05 May 2014 5,980 5 View

Does anyone have experience with biomass (Lignocellulose) pre-treatment?

I am working on bioethanol production. How can I quantify the reducing sugars percentage (glucose, xylose, arabinose,galactose, etc.) by using HPLC, after pretreatment, and what are the column...

07 July 2013 2,343 5 View