Hello Every one,
I am working on pretreatment of lignocellulosic biomass, I am facing a problem while preparing a smear for SEM analysis of pretreated sawdust. please let me know the smear preparation in-detail and suggest me one of the best methods.
Thanking you All.
Easiest way (may work for you): put sticky carbon tape on specimen stub, press it in sawdust, knock out excess of the dust (with hard knocks on a table), apply conductive coating, put it in SEM.
Sawdust should be dry; if needed let it air dry for a while.
For conventional SEM the Vladimir suggestion is perfect however, if you has acces to the SEM with Low vacuum mode, the mentalization and the drying are not required.
For specimens like wood conventional (not environmental) SEM will provide better images of better quality.
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