Hello. I am having some experiment about differentiation periosteal-derived cells to osteoblasts in 2D culture. I cultured cells in OM (osteogenic medium) and additionaly certain amount of BMP-2 was added in medium.
After 7, 14, 28 days I checked ALP activty and calcium deposition. ALP was increased until 14days, but calcium was not detected until 28 days. Plus I did RT-PCR, but late marker (osteocalcin, osteopontin) was not detected.
Has anyone eperienced about osteogenic undifferentiation, please answer.
Thank you for reading and sorry for my bad english.