How does formic acid elute the peptides for the polyacrylamide gel after trypsin digestion?

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Alaa Jabbar Al-Manhel

Hi , i agree with Andreas Schmidt answers ...good luck.

Siddharth Bhatia

Hi Andreas

Very well explained. But if you are saying proteins have hydrophobic interactions with acrylamide, then at the destaining step when we use acetonitrile to remove CBB, protein should also elute out. But its not the case. So what is happening at that time?