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Questions related from Siddharth Bhatia
I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...
09 November 2023 9,094 0 View
I have performed venom fractionation using HPLC and run the samples in SDS PAGE. I wanted to know what could be the possible reason for my protein bands to look like this. It should have been...
16 March 2023 3,505 6 View
I am working on snake venoms and we have to routinely check PLA2 activity. I am looking for a non-kit based method that can be done easily. Thanks
11 December 2021 8,655 3 View
I am working on a peptide that inhibits venom toxin activity. I wanted to know how the peptide is able to have inhibitory effects on venom proteins. To do that I need to figure out what proteins...
16 June 2021 1,987 9 View
I have isolated peptides from venom proteins and I would like to identify it by matching it to database. Since there is no genome sequence for my snake species, I would like to use database that...
09 January 2019 9,235 3 View
My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
08 May 2018 5,516 1 View
I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?
16 January 2018 1,222 1 View
My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?
05 January 2018 2,682 6 View
I am performing protein fractionation using RP HPLC. I wanted to do relative abundance of protein present using area % as a measure. But the software is not able to collect accurate peak starting...
14 December 2017 4,878 5 View
I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?
11 February 2016 2,397 2 View
I want to check the expression of hsp70 in frogs by qPCR method. For that i have construct primers of conserved sequence as i want them to work in all species of frogs
12 June 2015 6,812 1 View
I am performing in-gel digestion. I wanted to know exact chemistry of the process by which 100% acetonitrile dries up the gel and turn it yellow.
01 January 1970 6,455 4 View
I have to perform LD50 experiments. For that I need accurate concentration of venom to be known.
01 January 1970 6,246 5 View
I am performing in-gel digestion for MS analysis. We use 5% Formic acid to elute the peptides into the solution. I wanted to know the mechanism of this process.
01 January 1970 1,431 2 View
