10 Questions 8 Answers 0 Followers
Questions related from Siddharth Bhatia
I have performed venom fractionation using HPLC and run the samples in SDS PAGE. I wanted to know what could be the possible reason for my protein bands to look like this. It should have been...
16 March 2023 3,489 6 View
I am working on snake venoms and we have to routinely check PLA2 activity. I am looking for a non-kit based method that can be done easily. Thanks
11 December 2021 8,646 3 View
My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
08 May 2018 5,495 1 View
I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?
16 January 2018 1,202 1 View
My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?
05 January 2018 2,667 6 View
I am performing protein fractionation using RP HPLC. I wanted to do relative abundance of protein present using area % as a measure. But the software is not able to collect accurate peak starting...
14 December 2017 4,866 5 View
I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?
11 February 2016 2,384 2 View
I am performing in-gel digestion. I wanted to know exact chemistry of the process by which 100% acetonitrile dries up the gel and turn it yellow.
01 January 1970 6,444 4 View
I have to perform LD50 experiments. For that I need accurate concentration of venom to be known.
01 January 1970 6,232 5 View
I am performing in-gel digestion for MS analysis. We use 5% Formic acid to elute the peptides into the solution. I wanted to know the mechanism of this process.
01 January 1970 1,419 2 View
