How does calcium work in retinal microglial cells?

More Sarpras Swain's questions See All

Potential Vs current density(mA/cm-2) in EC-Lab?

In Zn-Air Battery, how to plot Potential vs current density(mA/cm-2) in EC-Lab softare/BioLogic instrument. Please suggest. plot of the ref....

04 July 2024 3,807 2 View

What should i use to align molecule for QSAR?

I have molecules with different groups. Some are bis chalcones, some are Dicoumarins, etc. How do I align them in Sybyl software if I can't select any common template?

27 June 2024 7,085 1 View

How to plot Potential vs current density(mA/cm-2) ?

In Zn-Air Battery experiment, how to plot Potential vs current density(mA/cm-2) from discharge voltage curves. Please suggest.

23 June 2024 860 2 View

What method or machine learning algorithm can be used?

I have the molecule's activity value and molecular descriptors. How can I find out which descriptors are the main components for increasing the activity of molecules? also, what Machine learning...

29 May 2024 4,169 2 View

What could be the problem of tz-mbl assay?

I am doing Tz-mbl assay(beta-galactosidase assay) for evaluating anti-viral activity for hiv infection. When I am doing with p24 assay is coming good but when I am doing it in tz-mbl assay I am...

22 September 2023 5,377 0 View

What assay can I perform?

I want to check inhibition activity of small molecules against HIV-1 entry. What assay I can perform other than fusion assay?

17 September 2023 5,190 4 View

Could microplastics in soil introduce drug-resistant superbugs to the food supply?

Any one can explain about this

25 August 2023 5,617 1 View

What are other procedure for antiviral assay for HIV?

I want to perform an antiviral assay for HIV inhibitors. I can't do the p-24 assay and I can only do the beta-galactosidase assay. So which cell lines can I use for this other than the tz-mbl cell?

16 July 2023 6,856 2 View

Optical and acoustic mode in FMR ?

We have observed in literature regarding the optical mode and acoustic mode in FMR spectra. How to identify which one is acoustic and which one is optical? Kindly clarify.

07 June 2023 3,987 3 View

Data analysis problem?

I have two different data table of molecular descriptors of small molecules according to their activities. I want to compare those two datasets and see if there is anything common features between...

01 May 2023 8,658 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Ross Hamilton

Calretinin might be an interesting protein to look at. I used it along with CD11b to image the microglia. It was back in 2007 and I think there was a difference in calretinin expression in STZ rats

Marianne Levon Shahsuvaryan

I hope that my written papers " Glaucomatous optic neuropathy: role of neuroprotection " and others find in Research gate wil be helpful. Good luck !