We used a specific activity inhibitor (chemical)to a particular gene to see changes in its functional alteration. But we found that after inhibitor treatment, its expression also gets reduced. Can anyone tell me the possible reason?
Dear Dr.Madhurima Das
In such a case, reduction in mRNA levels could be because of changes in the promoter activity. After the inhibitor treatment, the expression of mRNA reduces which is suggestive that the gene expression is being regulated at the promoter level. Maybe the inhibitor blocks the attachment of RNA polymerase to the promoter, thus preventing transcription of the gene into mRNA.
As you very well know that a promoter is a region of DNA upstream of a gene where relevant proteins (such as RNA polymerase and transcription factors) bind to initiate transcription of gene to produce mRNA.
Regards,
Malcolm Nobre
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