01 January 1970 5 2K Report

I am performing in-gel digestion. I wanted to know exact chemistry of the process by which 100% acetonitrile dries up the gel and turn it yellow. 

More Siddharth Bhatia's questions See All
Which proteomics software is best for performing IBAQ analysis?

I have protein samples which I need to identify. I loaded it on SDS PAGE. Now each band may contain two or more proteins. Can I estimate relative abundance of those proteins through IBAQ? If yes,...

05 June 2019 6,131 3 View

Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

04 May 2018 1,828 1 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

31 December 2017 3,059 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

31 December 2017 6,581 8 View

Why do I get smear on SDS-PAGE?

I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?

04 May 2016 9,119 5 View

What is polymorphic marker?

I was reading an article where they have mentioned alleles of polymorphic markers. they are talking about U2 tandem arrays. 

02 March 2016 8,035 2 View

How does DNA get compressed in stacking gel?

I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?

01 February 2016 7,311 2 View

After running SDS, why do we put our gel in solution of acetic acid and methanol?

I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.

01 February 2016 506 5 View

Can anyone give me examples of gene-lists and gene-sets used in GSEA?

I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique

09 October 2015 7,558 4 View

How to do northern blotting to check expression of HSP proteins in frog ?

I have to quantify expression of HSP proteins in frog. So what could be best possible technique to do that? 

04 May 2015 5,343 3 View

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