Did I perform my glm right?

Some of you might recognize my question and the background, but I struggle with this issue for more than a month now and there is only little progress... Here some background info: I have a...

09 October 2020 4,611 7 View

Which test to use when comparing skewed proportional data between two groups?

Well... the title covers the whole problem. I have very skewed proportional data (the hatching success of a iguana. NOTE that it is a proportion for every nest. e.g. for nest N1, 9 hatchlings...

10 September 2020 3,208 4 View

How to perform and interpret a GLM on skewed proportional data?

Hi there, I know I already posted some questions on this issue, but I still cannot perform this GLM according to expectations. First, I have a dataset with multiple explanatory variables (e.g....

09 September 2020 8,870 8 View

Two random factors in a Linear Mixed Model and Generalized Linear Mixed Model in R?

Hi there! A short summary of my data: I have song recordings of 60 birds (multiple recordings per bird). During the research period, some birds had more than one nest. Now I want to find out...

17 August 2020 1,322 3 View

Troubleshooting rAb expression?

Hi All, I'm trying to express a rAb in CHO or HEK293 cells and I am having an issue getting functional antibody. WB shows bands for HC and LC under reducing conditions and non-reduced shows a...

10 July 2019 8,613 4 View

Will thrombin aptamers bind immobilized/denatured thrombin?

I'm interested in modifying a polyacrylamide gel-based immunoassay to use aptamers in place of antibodies. In this assay, the proteins are covalently immobilized within the polyacrylamide gel via...

15 May 2018 5,490 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View