hi :

i think this study can help you

Shalayel M.H.F., Elhussein A.B., Fadlelseed O.E.,

Abdelgadeir A.E.A., Naeem A., Elsheikh O.M., 2014.

Platelets malondialdehyde levels in thrombocytopenic

malaria patients caused by Plasmodium falciparum. The

Journal of Physiology and Health. Photon 110, 305-309

Thank you. Can you point out to me where in the article there is reticulocyte separation? I did not find any mention of it

It depends on the level of purity and number of reticulocytes that you need. Percoll gradients can be tricky to get good separation.  Most reticulocytes express CD71, so you could use magnetic bead separation with anti-CD71. 

ok. Would you suggest separating the blood first into different fractions (rbcs, wbcs, plasma) and then probing for platelets? 

Yes, removal of WBCs and platelets is best so that you have a cleaner population of RBCs to start with. Make sure that you have a good separation of the WBC and RBC  fractions and do not disturb the RBC layer as most of the reticulocytes will be at the top. 

Hi Jada, I am currently looking for reticulocyte isolation protocols. How did your project go? Were you able to establish a good protocol? thank you!

https://smartech.gatech.edu/bitstream/handle/1853/58256/BANTON-DISSERTATION-2017.pdf I hope this could help you