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Questions related from Jada Selma
I am trying to inhibit the phosphorylation of a Erk 1/2 but the cells I am working with naturally have it already phosphorylated. I want to see if I can remove the phosphate group with a specific...
01 December 2014 8,033 3 View
I am trying to stain cholesterol in THP-1 cells, but I have heard the Fillipin III photobleaches very quickly and it is hard to see in under confocal
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I am trying to label a phosphorylated protein in one cell type and then image if the protein is transferred to another cell type after co-culture.
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Has anyone ever mounted naive THP-1 cells on coverslips for confocal imagining? I want to image THP-1 cells without polarizing them to M0 macrophages, but I have had problems getting them to...
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I am attempting to isolate neutrophils from human blood samples. From most protocols I have seen, the process requires 4% Dextran and using Histopaque-1077. Is the Dextran necessary? I want to do...
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