5 Questions 10 Answers 0 Followers
Questions related from Jada Selma
I am trying to inhibit the phosphorylation of a Erk 1/2 but the cells I am working with naturally have it already phosphorylated. I want to see if I can remove the phosphate group with a specific...
01 December 2014 8,031 3 View
I am trying to stain cholesterol in THP-1 cells, but I have heard the Fillipin III photobleaches very quickly and it is hard to see in under confocal
10 November 2014 567 2 View
I am trying to label a phosphorylated protein in one cell type and then image if the protein is transferred to another cell type after co-culture.
08 November 2014 4,353 3 View
Has anyone ever mounted naive THP-1 cells on coverslips for confocal imagining? I want to image THP-1 cells without polarizing them to M0 macrophages, but I have had problems getting them to...
08 July 2014 372 10 View
I am attempting to isolate neutrophils from human blood samples. From most protocols I have seen, the process requires 4% Dextran and using Histopaque-1077. Is the Dextran necessary? I want to do...
29 June 2014 2,682 25 View