Jada Selma

6 Questions 12 Answers 0 Followers

Questions related from Jada Selma

How do you separate reticulocytes from whole blood?

I want to isolate reticulocytes from blood to further characterize them for a study. Can I use a Percoll or Ficoll gradient?

13 April 2015 6,803 7 View

Can you purchase specific phosphatases like you can inhibitors?

I am trying to inhibit the phosphorylation of a Erk 1/2 but the cells I am working with naturally have it already phosphorylated. I want to see if I can remove the phosphate group with a specific...

01 December 2014 8,086 3 View

Is there another stain besides Filipin III for cholesterol?

I am trying to stain cholesterol in THP-1 cells, but I have heard the Fillipin III photobleaches very quickly and it is hard to see in under confocal

10 November 2014 635 2 View

Is it possible to put a fluorescent tag on a protein in order to track if its transferred to neighboring cells ?

I am trying to label a phosphorylated protein in one cell type and then image if the protein is transferred to another cell type after co-culture.

08 November 2014 4,408 3 View

How can I get suspension cells to adhere to the coverslips?

Has anyone ever mounted naive THP-1 cells on coverslips for confocal imagining? I want to image THP-1 cells without polarizing them to M0 macrophages, but I have had problems getting them to...

08 July 2014 408 10 View

Does anyone know a protocol for primary human neutrophil isolation?

I am attempting to isolate neutrophils from human blood samples. From most protocols I have seen, the process requires 4% Dextran and using Histopaque-1077. Is the Dextran necessary? I want to do...

29 June 2014 2,724 25 View