How do you run RT-PCR?

02 November 2022 0 645 Report

I am trying to run RT-PCR of brain sample and I was wondering what is a good RT-PCR step by step protocol, from brain tissue collection to the data analysis/imaging I could use, thank you.

Badges
Science topic

More Linda Zoungrana's questions See All

Does anyone have a protocol for fixed Brain tissue co-staining with TUNEL?

I have In Situ Cell Death Detection Kit from Roche and wanted to stain fixed brain tissue (not paraffin embedded tissue, just frozen section)with two other proteins. Does anyone have the protocol...

07 March 2024 2,441 0 View

Why are the Kinase and Ligase activities not required for the overlapping mutagenic primer strategy? Why is DpnI step still required?

For PCR primer. I was wondering Why are the Kinase and Ligase activities not required for the overlapping mutagenic primer strategy? Why is DpnI step still required (or at least helpful)?

08 September 2022 10,019 2 View

How to calculate volume for a serial dilution?

I am conducting a serial dilution of antibody for an ELISA protocol consisting of 12 well-plate I need a final volume of 300 uL and performing a 1/5 dilution what will be my initial volume and the...

02 April 2020 7,419 3 View

How and when should I feed malaria parasites culture?

I am currently taking care of Plasmodium falciparum in vitro culture and in order for me to keep them alive, I was wondering how and when should I feed them if you have any protocol to advice me....

19 February 2020 1,849 5 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View