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Questions related from Linda Zoungrana
I have In Situ Cell Death Detection Kit from Roche and wanted to stain fixed brain tissue (not paraffin embedded tissue, just frozen section)with two other proteins. Does anyone have the protocol...
08 March 2024 2,401 0 View
I am trying to run RT-PCR of brain sample and I was wondering what is a good RT-PCR step by step protocol, from brain tissue collection to the data analysis/imaging I could use, thank you.
02 November 2022 586 0 View
For PCR primer. I was wondering Why are the Kinase and Ligase activities not required for the overlapping mutagenic primer strategy? Why is DpnI step still required (or at least helpful)?
09 September 2022 9,977 2 View
I am conducting a serial dilution of antibody for an ELISA protocol consisting of 12 well-plate I need a final volume of 300 uL and performing a 1/5 dilution what will be my initial volume and the...
03 April 2020 7,373 3 View
I am currently taking care of Plasmodium falciparum in vitro culture and in order for me to keep them alive, I was wondering how and when should I feed them if you have any protocol to advice me....
20 February 2020 1,837 5 View