I have done PCR for gene expression analysis, after getting PCR I have done Band analysis on image lab5. How can put a statistics?
Just a suggestion, I don't think your densitometric analysis of an endpoint PCR reaction is worth a quantification... at least you should make a cycle titration and avoid the saturation cycles (loading on a gel the same reaction stopped in a series of consecutive cycles you'll see your product increase and at some point remain constant... that's your saturation point, and it might be or should be different in your different conditions: choose a cycle where you are not on saturation in all your conditions). Still, qPCR would be more appropriate...
Then, if you want to graph your measurements, you should have at least n=3 of cDNAs (or extracted RNAs) for each condition you are analysing (so, 3 independent biological samples, not triplicates from one cDNA) and you can average them and calculate the standard deviation. Many softwares can help you with this, GraphPad Prism is one, also Excel will do it. Statistical analysis will depend on the design of your experiment. If you have only two conditions you can use a Student's T-test (on excel, but also on line, for example: http://www.graphpad.com/quickcalcs/ttest1/), but if you have more it will be safer to perform an ANOVA... if you are not familiar with statistics you'd better ask somebody who knows how to do it.
Can you show us the plots you have made, and tell us what question you are trying to answer. It's not possible to recommend how to display data or to test it statistically unless you tell us the question.
plug in the values in graph pad prism software and follow instructions, the s/w does everything for you.
Just a suggestion, I don't think your densitometric analysis of an endpoint PCR reaction is worth a quantification... at least you should make a cycle titration and avoid the saturation cycles (loading on a gel the same reaction stopped in a series of consecutive cycles you'll see your product increase and at some point remain constant... that's your saturation point, and it might be or should be different in your different conditions: choose a cycle where you are not on saturation in all your conditions). Still, qPCR would be more appropriate...
Then, if you want to graph your measurements, you should have at least n=3 of cDNAs (or extracted RNAs) for each condition you are analysing (so, 3 independent biological samples, not triplicates from one cDNA) and you can average them and calculate the standard deviation. Many softwares can help you with this, GraphPad Prism is one, also Excel will do it. Statistical analysis will depend on the design of your experiment. If you have only two conditions you can use a Student's T-test (on excel, but also on line, for example: http://www.graphpad.com/quickcalcs/ttest1/), but if you have more it will be safer to perform an ANOVA... if you are not familiar with statistics you'd better ask somebody who knows how to do it.
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