Once you pick them from the sediment, is it ok just to freeze them or do you need to use a specific solution for preservation?
Alcohol 96% or higher is the best solution to preserve specimens for posterior DNA analysis.
Thanks a lot! I've read of a DNA buffer, do you know if its just alcohol 96% or anything else?
Well, I never read about a DNA buffer. However i used alcohol for the preservation of corals, sponges, moluscs, hydrozoan, polychaetes and also samples of blue shark. Then I or other researchers used that samples in the lab and it was possible to amplify them for DNA Barcoding, sequencing of other genes or genotyping.
Yes, you can use EtOH 96% and if your organisms are benthic invertebrates you can also add 5% of glycerin of the EtOH volume used that better preserves the organisms.
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs