Hello,
I have to synthesize a promoter sequence, but due to Long homopolymer stretches (AAAA...), it is impossible to do it. Can I change the sequence with codon usage without affecting the promoter function in the translation?
Do you ever have precipitation problem during preparation?
29 November 2023 3,875 2 View
Hello, Have you ever extracted nuclei from fixed samples ? When I fix my HepG2 cells with 1% formaldehyde, I noticed that the fixation prevents cell lysis with regular nuclei isolation buffer,...
03 May 2023 561 5 View
Hi, I have a dyadic dataset with distinguishable dyads (husbands and wives). I want to test a model with a predictor (X(f) X(m)), a mediator which is a similarity/difference in a trait, and...
14 January 2021 9,642 1 View
Hello all, Some time ago I solved a transient thermo-mechanical problem using Abaqus 6.10, and I was quite satisfied with the observed results (frictional dissipation energy and power loss due to...
28 December 2017 1,153 4 View
I have a latent growth curve model in AMOS (4 points of time) and constructed the slope and intercept. However, when I try to add observed predictors to the slope or intercept the program does not...
12 May 2015 8,003 6 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
I have 2 papers below, but I can't access this, you can help me? Shuai Zhang, Xiaodi Li, Xingyu Zhou, Yuning Wang, Yue Hu, Cloud removal using SAR and optical images via attention mechanism-based...
18 July 2024 9,635 0 View
My supposition or question: Is the 600 tetrahedron cell {3,3,5} polytope a four dimensional quasicrystal? I do think so, because its circumsphere is described by a radius of exactly the golden...
16 July 2024 4,653 0 View
DEAR COLLEAGUES!We invite you to take part in the InternationalConference "Intelligent Transport Systems:Ecology, Safety, Quality, Comfort", which will beheld on November 26–27, 2024 Available...
10 July 2024 8,173 3 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
When considered the natural environment and the habitus of the translators, it can be seen that in the end of the day they are under the absolute and inevitable dominance of the cultural norms...
07 July 2024 4,717 1 View
I'm writing a letter of recommendation for a colleague and I'd like to mention how often her publications are cited by other academics.
28 June 2024 4,995 0 View
I need the exact method to determine the letters a, b, and c noted in the exponent of the standard deviation value of color, for example, like in the picture added. The letters are marked with yellow.
24 June 2024 4,927 1 View