How to measure pH in tumors and the tumor micro-environment of animals during the experiment?
The only thing I can think of is to use fluorescent labelled abs that specifically bind the tumor that change color with pH ( things like SNARFS>. The issue would be can you see the fluorescence through the skin of the tumor. Another route is infrared beacons, that could be made to possibly " light up" under certain pH conditions. I would get in touch with Lynn Matrisian, another person on Researchgate. Her work on beacons is exemplary but I don't know if any she has made change or react differently if the pH of the tumor changes the micro environment. But I would think they could be engineered to do this.
Dear Terezia, dear Marcia,
not working in this specialized field but interested in the practical solution of such problem (in terms of ultrastructural evaluation) I remember the following:
[highly sophisticated] 2D high-resolution imaging of pH in vivo: a ratiometric sensing scheme is based on time-domain luminescence imaging of FITC and ruthenium(II)tris-(4,7-diphenyl-1,10-phenanthroline).
Title:
Stephan Schreml et al, PNAS February 8, 2011 vol. 108 no. 6, pp. 2432–2437
(at least abstract: www.pnas.org/cgi/doi/10.1073/pnas.1006945108 = http://www.pnas.org/content/108/6/2432. If you need the pdf for reading and cannot download it, please just send me a note either via ResearchGate internal messaging system or by writing to my personal mail-address [email protected])
In their introduction they state: “
An other option to that 2D HR-imaging model (as Marcia pointed out) would be studying cellular pH using pH-sensitive fluorescent dyes [SNARFS], e.g. cf. also
Hélène A. Benink et al,
BioTechniques, Vol. 47, No. 3, Sept 2009, pp. 769–774
(should be open access at: http://www.biotechniques.com/multimedia/archive/00055/BTN_A_000113220_O_55818a.pdf)
It would be interesting too to get a little bit more detail on the outline of Terezia's study (in vivo IN the animals during experiment?, cell culture? etc.).
Not knowing about further articles specifically dealing with intracellular/extracellular pH-Measurement in vivo/cell-Tumor cultures, but interested though,
with best wishes and regards, Wolfgang
Notice added:
perhaps you've found the other posting thread in ResearchGate: Jan Trnka's question:
How do you convert SNARF fluorescence ratios to pH values (intracellular, confocal)?
see: https://www.researchgate.net/post/How_do_you_convert_SNARF_fluorescence_ratios_to_pH_values_intracellular_confocal?ch=reg&cp=re221_aq_p33&pli=1&loginT=diZWglsws5_nUlrfzkPcpeLCHF1KSJmz
Perhaps additional information given, best regards, Wolfgang
Sorry, I worked on intracellular pH of tumor cells rather than that of tumor environment. However, I could find for you some papers which, I hope, will help you (old and new methods).
I use SNARF in a Dorsal Window Chamber (DWC) model to measure xenografted tumor pH and it's microenvironment. If you can do DWC in your institute I will send you our protocol?
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